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KMID : 0545120000100030394
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 3 p.394 ~ p.398
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Falcarindiol,a Polyacetylenic Compound Isolated from Peucedanum japonicum,Inhibits Mammalian DNA Topoisomerase I
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LEE, GWANG
PARK, HYOUNG-GUN/CHOI, MI-LIM/KIM, YOUNG HO/PARK, YONG BOK/SONG, KYUNG-SIK/CHEONG, CHAEJOON
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Abstract
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A methanol extract of the root of Peucedanum japonicum, used as a medicinal herb, showed an inhibitory effect on mammalian topoisomerase Ⅰ activity. The methanol extract was suspended in ethyl acetate, and a topoisomerase Ⅰ inhibitor in the organic soluble fraction was then isolated by silica gel and thin layer chromatography. The topoisomerase I inhibitory compound was identified as falcarindiol based on the analysis of EI-MS, ^1H and ^13C NMR spectroscopy. This inhibitor showed cytotoxicity against human leukemia Jurkat T and HL60 cells with an IC_50 value of 7㎍/㎖. These results suggest the possibility of falcarindiol as a new anticancer agent which can be expected to have a synergistic effect on other anticancer drugs. In addition, the present data show that falcarindiol has antifungal, yet not antibacterial, activity.
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KEYWORD
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